Work. Yay.

[quesarah]

It's insanely busy. I've started the process of evaluating a bunch of test primer sequences; Frenchie wants to see how they behave when they're labeled with different fluorescent dyes. So I have 48 primers, each of which is labeled with 4 dyes. That's 192 primers that need standard curves with each standard curve performed on 7 log dilutions of template plus No Template Controls in triplicate. Given that I can run 16 standard curves per 384 well plate, this means I will need to run 12 full 384 well plates to complete the experiment.

The first is running. The second will run this afternoon. Maybe I can squeeze in a third, but I think I'm supposed to be in a meeting from 3:30-4:30. (Whoopie.)

This will take a long time.

Comments

[virginmartyr1.livejournal.com]

this is what i read:

It's insanely busy. I've started the process of evaluating a bunch of test primer sequences; Frenchie wants to see how they behave blah blah blah blah blah blah something something blah something florescent blah blah something else

but i got the main idea, which is that you're super insanely busy. but that i already knew. the science stuff went right over my head. whoosh.

[biogeekgrrl.livejournal.com]

re: what you read:

Yeah, that pretty much sums it up.